Reporter Genes. A Practical Guide
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Author(s): D. S. Anson (Ed.)
Publisher: Humana Press
ISBN: 9781588297396
Format: hardback + CD-ROM
158pp
Price: $99.50
Review Date: 27 November 2007
Review: Reporter genes play a vital role in many areas of biological research by providing a means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. The eleven chapters describe practical protocols for experimentation with the available most useful reporter genes for mammalian system, concentrating on the most commonly used marker genes. Topics include: staining and visualisation of ?-galactosidase in mouse embryos and tissues; immunohistochemical detection of ?-galactosidase; reporter gene expression in murine airways; 3D analysis of molecular signals with episcopic imaging techniques; fluorescent proteins for cell biology; GFP detection in Drosophila melanogaster; autofluorescent proteins for flow cytometry; fluorescent protein reporter systems for single-cell measurements; subcellular imaging of cancer cells; imaging of molecular events with bioluminescent reporter genes; green fluorescent protein as a tracer in chimeric tissues. The accompanying CD contains illustrations from the book.